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KingsVision™ Bioluminescence/Fluorescence Small Animal In Vivo Imaging System

KingsVision™ Imaging Instrument for Dual Modality Bioluminescence and Fluorescence with Novel NIR-I and NIR-II Dyes and Probes

KingsVision dual modallity NIR-II/SWIR fluorescence/bioluminescence in vivo imaging: 400nm- 1700nm window, ~cm penetration

KingsVision™ instrument provides users an additional modality of bioluminescence imaging in addition to the fluorescence imaging capability described in DeepVision™.

(Also see details about DeepVisionTM. Also see Nirmidas NIR-I and NIR-II dyes and probes.)

In addition to the features offered in DeepVisionTM, additional Key Features of KingsVisionTM :

  •   Bioluminescence imaging modality with a second high quality camera 

  •        High performance and low noise CCD camera. 

  •        Very low dark current and read out noise

  •        High quantum efficiency

  •        Small pixel size

KingsVision™ Imaging System Specification

Imaging System Components




Camera1 Active Area

13 mm x 13 mm< 10 mm x 8mm

Imaging Pixels

1024 x 1024

640 x 512

Quantum Efficiency

80%-95% in 0.4-0.9 µm

80%-90% in NIR-II 1.0-1.7 µm; 

50-80% in 0.8-1.0 µm

Pixel Size

13 µm x 13 µm

15 µm x 15 µm

Minimum Field of View (FOV)

1.0 mm x 0.75 mm (microscopy mode)

Maximum Field of View (FOV)

62 mm x 50 mm (whole body mode, up to four mice)

Highest Spatial Resolution

< 2.5 µm

< 5.0 µm

Stage Temperature

20 °C – 40 °C, heated stage. 37°C by default.

Power Requirements

110 or 220 V, 50 Hz – 60 Hz

Dark Current

< 0.01 e-/p/sec

< 1500 e-/p/sec

Read Noise

< 3 e-  

< 20 e(High Gain)  

<180 e-  (Low Gain)  


808 nm and 975 nm (for NIR-II and upconversion imaging)

Emission Filters3

700 nm shortpass (for upconversion imaging)

1100 nm long pass, 1300 nm long pass and 1500 nm long pass

Camera Operating Temperature

-60 °C to -100 °C

-15 °C to -30 °C

Imaging System Space Requirements

61*60*96 cm (W x D x H)

Imaging Modes

NIR-I imaging, NIR-II imaging, Bioluminescence imaging, Upconversion imaging, Lifetime imaging

Lifetime Imaging Mode

User specified laser excitation time, laser off, followed by specified exposure/imaging time

1. Nirmidas uses a new camera technology with superior performance, allowing for much shorter exposure time and lower background.

2. Different laser lines are available upon request

3. Different filters are available upon request

Application Examples 

KingsVision™ image result combining both NIR-IIb fluorescence imaging and bioluminescence imaging. 

Example of KingsVision image:rare-earth down-conversion nanoparticles dye/luc2 to study 4T1-luc tumor and mouse blood vessle

Caption: Images obtained by Nirmidas KingsVisionTM . NIR-IIb imaging of circulating rare-earth down-conversion nanoparticles (Nirmidas Product No. NBDY-0029A) in mouse blood vessels and bioluminescence image of 4T1-luc tumor. 

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