Click here for Products

NIR-II Dye and NIR-I-II Dye


NEAR INFRA-RED DYE TECHNOLOGY


Fluorescence-based optical imaging is indispensable to investigating biological systems with high spatial resolution and fast temporal speed. Combined with Nirmidas DeepVisionTM NIR-II fluorescence imaging instrument and KingsVisionTM dual modality fluorescence and bioluminescence imaging instrument, our cutting-edge infrared dyes will empower research for vasculature, tumor, brain, lymphatic/lymph node imaging and molecular imaging. 

Imaging in the second near infra-red window (NIR-II), beyond 1000 nm, is promising for deep-tissue high resolution optical imaging in vivo:


  • Compared to visible and near infra-red (below ~900 nm), the NIR-II window enables reduced scattering of photons traversing through tissues

  • NIR-II optical window imaging can resolve micron scale features at 3-5

    millimeter depth through the intact body and brain of mice.  

  • High speed/frame rate imaging allows for blood-flow speed mapping in

    individual capillary vessels, capable of resolving flow patterns within a

    single cardiac cycle of mice

  • Non-invasive brain imaging can be achieved without craniotomy

Imaging of mouse brain vasculatures using quantum dots (IR-iNP

image of mouse brain vasculatures using quantum dots by the group of professor Hongjie Dai, Stanford University

Nirmidas’ NIR-II fluorophores are of high quantum yield and can be functionalized by a variety of targeting moieties for molecular imaging and vessel/organ imaging in vitro and in vivo.

Comparison of NIR-I and NIR-II probes for mouse organ/tissue molecular imaging: ischemic mouse hindlimb imaging


NIR-II DYE/PROBE PROPERTIES

Nirmidas has developed a series of near infra-red II (NIR-II) dye products suitable for in vivo, ex vivo and in vitro imaging applications. These organic dyes and inorganic probes exhibit the following characteristics.

  • Large Stokes' shift.

  • Excitation (700 nm-1000 nm); emission (1000 nm-1800 nm).

  • High quantum yield/brightness.

  • Low toxicity to cells in vitro and to small animals in vivo.

  • Blood circulation halftimes ~ 1-5 hours, tunable.

  • Rapid excretion from body.

  • Can be conjugated to antibodies, peptides, aptamers and other ligands for molecular imaging and vessel/organ imaging in vitro and in vivo.


NIR DYE APPLICATIONS

  • Fluorescence imaging/spectroscopy in the NIR-I window (800 nm-1400 nm)

  • Fluorescence imaging/spectroscopy in the NIR-II (SWIR) window (1000 nm-1700 nm)

  • Molecular imaging of specific biological targets in vivo and ex vivo.

  • Wide field, confocal and light sheet microscopy in NIR-II.

  • Intra-operative imaging

  • Image guided procedures

  • Photoacoustic imaging

  • Two photon imaging in NIR-II

  • Imaging, phototherapy and drug delivery; nanomedicine and theranostics.


Deep-tissue in vivo fluorescence imaging with various animal models for research and development in the research areas of:

  • Cancer

  • Brain/neuroscience

  • Cardiovascular diseases

  • Immune system




DYE/PROBE LIST

Research Use Only

1)      NIR-II dye. IR-E1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye. 

        (Product No. NBDY-0015)

  • Excitation: 650 nm-900 nm. Emission: 900 nm-1400 nm with peak ~ 1050 nm. 

  • Brightness 3 out of 5. 

  • Functional group: azide, NH2 or COOH. PEG containing, aqueous soluble. 

  • In vivo circulation time < 1 hour. Liver excretion.

  • Spectra:


    Spectrum of Nirmidas NIR-II dye. IR-E1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye. NBDY-0015. Liver excretion.



 2)      NIR-II dye. IR-T1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye.

          (Product No. NBDY-0020)

  • Excitation: 650 nm-900 nm. Emission: 900 nm-1400 nm with peak ~ 1050 nm. 

  • Brightness 3 out of 5.

  • Functional group: N3 azide. PEG containing, aqueous soluble. 

  • In vivo circulation time < 1 hour. Renal excretion.

  • Spectra:


    Spectrum of Nirmidas NIR-II dye. IR-T1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor. NBDY-0020. Renal excretion.



 3)      NIR-II dye. np-IR-m1050. Macromolecular, DLS size ~ 15 nm. 

          (Product No. NBDY-0022)

  • Excitation: 650 nm-900 nm. Emission: 900 nm-1400 nm with peak ~ 1050 nm. 

  • Functional group: NH2. PEGylated, aqueous soluble. 

  • Brightness 5 out of 5. Circulation time ~ 3 hours. Liver excretion.

  • Spectra:



    Spectrum of Nirmidas NIR-II dye. np-IR-m1050. Macromolecular, DLS size ~ 15 nm. NBDY-0015. Liver excretion.

* Reference: 

“A bright organic NIR-II nanofluorophore for three-dimensional imaging into biological tissues”, Hao Wan, et al., Nature Communications March 21, 2018 1171(2018);  https://www.nature.com/articles/s41467-018-03505-4

           



 4)      NIR-IIb probe. IR-iNP. Inorganic PbS/CdS core-shell quantum dots (QD), in PBS. DLS size ~ 20 nm. 

          (Product No. NBDY-0018A) 

  • Excitation: any wavelength shorter than emission wavelength. Emission peak ~ 1600 nm. 

  • Brightness 5 out of 5.

  • Functional group: NH2. PEGylated, aqueous soluble. 

  • In vivo circulation time ~ 5 hours. Liver excretion.

  • QD (PBS, 1mg/ml). Each unit: 1ml for $500

    Buy


       NIR-IIb probe. IR-iNP. Inorganic PbS/CdS core-shell quantum dots (QD), in Organic Solvent. DLS size ~ 20 nm. 

       (Product No. NBDY-0018B)

  • Default solvent: Chloroform. If you prefer nanoparticles in other organic solvent (Toluene, DCM etc.), please contact us by filling the inquiry form. 

  • QD (Chloroform, 5mg/ml). Each unit: 1ml for $500

    Buy


* Reference: 

“Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging”, Mingxi Zhang, et al., PNAS June 26, 2018 115 (26) 6590-6595;  https://doi.org/10.1073/pnas.1806153115


  • Spectra:

    Spectrum of Nirmidas NIR-IIb probe. PbS/CdS quantum dots (QD) based. Emission at 1600 nm, bright, liver excretion. NBDY-0018A




5)      NIR-II probe. HIPCO-CNT biocompatible aqueous suspension. Single-walled carbon nanotube based probe.

         (Product No. NBDY-0028)

  • Single-walled carbon nanotubes (SWNT). Diameter ~ 1 nm. Length ~ 200 nm-300 nm. 

  • Brightness 3 out of 5.

  • An ideal quantum yield reference standard. Non-photobleaching. Absolute QY~0.03% measured by integrated sphere for >1000 nm emission

  • Excitation: any wavelength in 650 nm-1000 nm. Emission ~ 1000 nm-1400 nm. 

  • Functional group NH2. PEGylated, aqueous soluble. 

  • In vivo circulation time ~ 3 hours. Liver excretion.


6)      NIR-I-II dye. IR-12N. IR-12N3 dye. Molecular MW: ~ 1,000 D. 

         (Product No. NBDY-0002)

  • Excitation: 700 nm-850 nm. Emission: 800 nm-1400 nm. Peak ~ 800 nm-850 nm. 

  • Brightness 5 out of 5. More stable and brighter than ICG. High molar extinction coefficient. Replacement for ICG, IRDye 800CW in vivo*.

  • Functional group: N3 azide group. Aqueous soluble. Refer to NBDY-0014 for a variant of this dye with NHS group is available upon request.

  • In vivo circulation time < 1 hour. Rapid liver excretion.


* Reference: 

1) “Repurposing Cyanine NIR‐I Dyes Accelerates Clinical Translation of Near‐Infrared‐II (NIR‐II) Bioimaging”, S Zhu et al., Adv. Mater; 2018. p. 10. 1002/adma.201802546. https://doi.org/10.1002/adma.201802546

 

2) “Near-Infrared-II (NIR-II) Bioimaging via Off-Peak NIR-I Fluorescence Emission”, S. Zhu et al.,

Theranostics. 2018; 8(15): 4141–4151. doi: 10.7150/thno.27995. http://www.thno.org/v08p4141.htm


  • Spectra:


    Emission, absorption Spectrum of Nirmidas NIR-I-II dye. IR-12N. IR-12N3 dye. Replacement for ICG, IRDye 800CW in vivo. NBDY-0002



7)      NIR-I-II dye. IR-12-NHS. Molecular MW: ~ 1,000 D. 

         (Product No. NBDY-0014)

  • Excitation: 700 nm-850 nm. Emission: 800-1400 nm. Peak ~ 800 nm-850 nm. 

  • Brightness 5 out of 5. Brighter than ICG. High molar extinction coefficient. Replacement for ICG, IRDye 800CW in vivo

  • Functional group: NHS group. Aqueous soluble. 

  • In vivo circulation time < 1 hour. Rapid liver excretion.


 8)      NIR-I-II probe. np-IR-12. Macromolecular size by DLS ~ 15 nm. 

          (Product No. NBDY-0026)

  • Excitation: 780 nm-820 nm. Emission: 800 nm-1400 nm with peak ~ 800 nm-850 nm. 

  • Brightness 5 out of 5. High molar extinction coefficient. Bright up to 1300 nm.

  • Functional group NH2 or others. PEGylated, aqueous soluble. 

  • In vivo circulation time ~ 3 hours. Liver excretion.


9)        NIR-IIb (1500-1700 nm) probe. IR-DCNP. Er based rare-earth down-conversion nanoparticle. 

           (Product No. NBDY-0029A)

  • Excitation: 980 nm. Emission: 1540 nm.

  • Brightness: 5 out of 5. 

  • Functional group: PEG-NH2 functionalized, aqueous soluble.

  • In vivo circulation time ~ 5 hours. Liver excretion. 

  • For Continuous wavelength and lifetime imaging. 

  • Nanoparticles soluble in organic solvents without transferred to aqueous phase are available upon request. Inquires please specify IR-DCNP-O (Product No. NBDY-0029B).


* Reference: 

“In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles”, Y. Zhong et al., Nature Biotechnology; 2019 Nov; 37 (11): 1322-1331. doi: 10.1038/s41587-019-0262-4. https://www.nature.com/articles/s41587-019-0262-4


  • Spectra:

    Spectrum of  NIR-IIb (1500-1700 nm) probe. Er based rare-earth down-conversion nanoparticle, lifetime imaging. NBDY-0029A



10)      NIR-I probe. np-NIR1-UV,  ultraviolet excitable. Macromolecular size by DLS ~ 15 nm.

           (Product No. NBDY-0013)

  • Excitation: UV-700 nm. Emission: 800 nm-1100 nm with peak ~ 900 nm. 

  • Brightness 5 out of 5.

  • Functional group NH2. PEGylated, aqueous soluble. 

  • In vivo circulation time ~ 3 hours. Liver excretion.


11)      NIR-I-II probe. AuMC (Gold Molecular Clusters)

           (Product No. NBDY-0039)

  • Containing ~ 25 gold atoms with glutathione (GSH) hydrophilic surface ligands. Cluster size <~ 3 nm 

  • Excitation: any wavelength shorter than ~900  nm. Emission range ~ 850-1350 nm

  • Brightness 4 out of 5.

  • Functional group: COOH and NH2. Aqueous soluble. 

  • In vivo circulation time < 1 hour. Renal excretion.


*Reference:

‘Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models’, Ani Baghdasaryan, Feifei Wang, Fuqiang Ren, Zhuoran Ma, Jiachen Li, Xueting Zhou, Lilit Grigoryan, Chun Xu, Hongjie Dai, 2022/9/24, Nature communications, Volume 13, Issue 1, Pages 1-11. https://pubmed.ncbi.nlm.nih.gov/36153336/


  • Spectra:

        Spectrum of Nirmidas NIR-I-II probe. Gold Molecular Clusters, non invasive, renal excretion, cancer models. NBDY-0039


Nirmidas NIR Dye is available to purchase today. Email us at info@nirmidas.com or fill in the form below.


Inquiry form     

Please fill out the following form to help us understand your needs better. One of our team members will reach out to you shortly.


This filed is mandatory.
This filed is mandatory.
This filed is mandatory.
This filed is mandatory.

看不清楚,点击刷新
This filed is mandatory.

Terms and Conditions of Sale

Technical Support and Customer Service